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KMID : 0380020140290030188
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Korean Journal of Biotechnology and Bioengineering
2014 Volume.29 No. 3 p.188 ~ p.192
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Sporulation of 7Lysinibacillus sphaericus7 WJ-8 Isolated from Concrete Pavement and Response to Environmental Stresses
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Han Sang-Hyun
Kang Chang-Ho
Shin Yu-Jin
Yeom Woo-Sung
Jeong Jin-Hoon
So Jae-Seong
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Abstract
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Calcite forming bacteria (CFB) have been received increasing attention as a novel and environmental friendly strategy for the healing of concrete crack. Among the CFB, spore forming bacteria were proposed to overcome concrete condition (high pH, hydration heat, deicer). In this study, Lysinibaclillus sphaericus WJ-8 (WJ-8) isolated from concrete pavement was characterized. The WJ-8 was able to precipitate calcite at 10 mg/mL. When observed by scanning electron microscopy, WJ-8 showed spore formation and maximum spore yield was approximately 97.9%. Also response of spores against various environment stresses was examined. Approximately 83~97% of spores maintained their survivability at each three conditions (60°C, 3 M NaCl and pH 12).
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KEYWORD
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Calcite forming bacteria, Lysinibacillus sphaericus, Calcite, Spore, Stress test
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